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SPOTLIGHT: Piper Longum Linn.

One of the core functions of our product development team, is to infuse into our formulas some of the most breakthrough blends known to man and science.

These herbs and extracts come from all over the world (Japan, China, India, Europe, Brazil, etc.), and have been expertly and thoughtfully combined into what we believe are the most superior products available on the market today.

Herbal Cleanse Tea is no exception, In fact, the ingredient list reads more like the ‘who’s who’ in the world of Ayurvedic medicine.

Over the weeks since its launch, we’ve featured stories on a couple of Herbal Cleanse Tea’s ingredients (Rooibos, Salacia Oblonga and Pu-erh tea).

Here’s another herbal spotlight that’s sure to get your attention: Piper Longum Linn.

From antioxidant, antiflammatory, antitumor and anticarcinogenic to neuroprotective and immunomodulatory, Piper Longum Linn is one powerful herb.

Checkout these abstracts from a few of the most recent studies on this one-of-a-kind herb.

Studies on the neuroprotective role of Piper longum in C6 glioma induced rats.

Subramanian U1, Poongavanam SVanisree AJ.


Many naturally occurring substances of plant origin ingested in human diet, exhibit anticarcinogenic and antimutagenic effects. One of the active phytochemical which shows the active anticarcinogenic role is Piper longum Linn. (Pl). Pl is widely used in ayurvedic industry due to its property in healing some of the bodily ailments. Despite being known for the antioxidant, antimicrobial and anticarcinogenic effects, its relation to brain and its tumour development is still scarce. Hence, the experimental glioma model was developed in rats using C6 glioma cells and the effect of Pl was evaluated in the brain tissue of experimental group of rats. From the study, the glioma induced animals showed an increased level of lipid peroxides (LPO), tissue marker enzymes lactate dehydrogenase (LDH), creatine kinase (CK), 5’nucleotidase (5’ND) and acetylcholine esterase (AChE). But Pl treatment (20 mg/kg body weight) significantly attenuated these alterations thereby showing potent anticancer effect in glioma induced rats. In addition, the anticarcinogenic effect of Pl was confirmed by microscopic analysis and the restoration of increased lipids and protein bound carbohydrates (PBCs) in the brain tissue of glioma induced rats. Hence our results implicate a major role for Pl in preventing the cancer development in the experimental glioma model.


Protective effect of Piper longum Linn. on monosodium glutamate induced oxidative stress in rats.

Thomas M1, Sujatha KSGeorge S.


Protective effect of ethanol extract of Piper longum Linn. against monosodium glutamate (MSG) induced toxicity was studied. Rats, orally administered with MSG at a dose of 8 mg/g body weight for 20 consecutive days, showed an increase in liver weight and rate of lipid peroxidation. Glutathione (GSH) in serum, liver and kidney showed decreased concentration. Significant increase was noticed in activities of serum alanine amino transferase (ALT) and aspartate amino transferase (AST), levels of serum triacylglycerol, total cholesterol and urea. Histopathological examination of liver and kidney showed central venous congestion, diffuse degeneration and necrosis of hepatocytes in para cortical and midzonal areas of liver and diffuse cortical tubular degeneration of kidney. Oral administration of ethanol extract of P. longum fruits at 300 mg/kg body weight along with MSG significantly reduced the levels of lipid peroxides in serum, liver and kidney, serum AST activity, serum levels of triacylglycerol and total cholesterol. Though, there was an increase in the level of GSH in tissues it was not significant. However, the treatment failed to reduce the levels of ALT and urea. Examination of tissue sections also exhibited normal histological architecture of both the organs. The present study revealed that administration of P. longum provided significant protection to liver and kidney from the oxidative stress of MSG, though the dose rate was not sufficient to provide a complete protection.


Pipernonaline from Piper longum Linn. induces ROS-mediated apoptosis in human prostate cancer PC-3 cells.

Lee W1, Kim KYYu SNKim SHChun SSJi JHYu HSAhn SC.


The antiproliferation effects of pipernonaline, a piperine derivative, were investigated on human prostate cancer PC-3 cells. It inhibited growth of androgen independent PC-3 and androgen dependent LNCaP prostate cells in a dose-dependent (30-90 μM) and time-dependent (24-48 h) manner. The growth inhibition of PC-3 cells was associated with sub-G(1) and G(0)/G(1) accumulation, confirmed by the down-regulation of CDK2, CDK4, cyclin D1 and cyclin E, which are correlated with G(1) phase of cell cycle. Pipernonaline up-regulated cleavage of procaspase-3/PARP, but did not change expression of proapoptotic bax and antiapoptotic bcl-2 proteins. Its caspase-3 activation was confirmed by the caspase-3 assay kit. In addition, pipernonaline caused the production of reactive oxygen species (ROS), increase of intracellular Ca(2+), and mitochondrial membrane depolarization, which these phenomena were reversed by N-acetylcysteine, a ROS scavenger. The results suggest that pipernonaline exhibits apoptotic properties through ROS production, which causes disruption of mitochondrial function and Ca(2+) homeostasis and leads to its downstream events including activation of caspase-3 and cleavage of PARP in PC-3 cells. This is the first report of pipernonaline toward the anticancer activity of prostate cancer cells, which provides a role for candidate agent as well as the molecular basis for human prostate cancer.


Immunomodulatory and antitumor activity of Piper longum Linn. and piperine

E.S SunilaG Kuttan


Alcoholic extract of the fruits of the plant Piper longum and its component piperine was studied for their immunomodulatory and antitumor activity. Alcoholic extract of the fruits was 100% toxic at a concentration of 500 μg/ml to Dalton’s lymphoma ascites (DLA) cells and 250 μg/ml to Ehrlich ascites carcinoma (EAC) cells. Piperine was found to be cytotoxic towards DLA and EAC cells at a concentration of 250 μg/ml. Alcoholic extract and piperine was also found to produce cytotoxicity towards L929 cells in culture at a concentration of 100 and 50 μg/ml, respectively. Administration of alcoholic extract of Piper longum(10 mg/dose/animal) as well as piperine (1.14 mg/dose/animal) could inhibit the solid tumor development in mice induced with DLA cells and increase the life span of mice bearing Ehrlich ascites carcinoma tumor to 37.3 and 58.8%, respectively. Administration of Piper longum extract and piperine increased the total WBC count to 142.8 and 138.9%, respectively, in Balb/c mice. The number of plaque forming cells also enhanced significantly by the administration of the extract (100.3%) and piperine (71.4%) on 5th day after immunization. Bone marrow cellularity and α-esterase positive cells were also increased by the administration of Piper longum extract and piperine.


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